Immunoaffinity Column as Cleanup Tool for a Direct Competitive Enzyme-Linked Immunosorbent Assay of Cyclopiazonic Acid in Corn, Peanuts, and Mixed Feed

Improved Direct Competitive Enzyme-Linked Immunosorbent Assay for Cyclopiazonic Acid in Corn, Peanuts, and Mixed Feed

An improved direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the analysis of cyclopiazonic acid (CPA) in corn, peanuts, and mixed feed. Two new approaches were used for the preparation of enzyme markers; one involved coupling CPA-bovine serum albumin (CPA-BSA) conjugate to horseradish peroxidase (HRP) using either the glutaraldehyde (GA) or the periodate (PI) me...

ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL

An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...

Enzyme-linked immunosorbent assay for detection of zearalenone in corn, wheat, and pig feed: collaborative study.

A direct competitive enzyme-linked immunosorbent assay (ELISA) screening method for zearalenone in corn, wheat, and feed at 500 ng/g was evaluated by 23 collaborators (22 laboratories) in an international collaborative study. Eighteen samples of spiked or naturally contaminated corn, wheat, and pig feed were prepared by the sponsoring laboratory and sent for testing with complete test kits to p...

Application of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep

Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for   epidemiologic studies. The present study was performed with a commercial human en...

Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition